Imaging of mouse experimental melanoma in vivo and ex vivo by combination of confocal and nonlinear microscopy

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  • 20 March 2012
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We investigated possibilities of confocal reflectance and  fluorescence microscopy in combination with SHG and two-photon autofluorescence imaging for the examination of experimental melanoma tissues in vivo, in mice under general anesthesia, and  ex vivo on freshly harvested specimens. Our approach facilitated  the examination of interrelationships between functional and  morphological aspects based on optical properties of the tissues. It proved to be suitable for describing the tumor structure and  documenting changes due to the effect of microwave hyperthermia treatment, such as modification of the collagen fiber network together with the other tissues. The results showed that the proposed approach could be suitable for a direct evaluation of  clinical interventions.